Suspension Time Kill Testing: ASTM E 2315 : Testing Facility Operation 026.2

Test Microorganism Test Substance Test Substance Concentration CFU/ml Percent Reduction Compared to Control at Time Zero Tot Kill
L. monocytogenes
15313
Control Control 2.30E+07 N/A
         KL-615 1 ppm 7.50E+05 96.74% 22,250,200
       KL-1112 1 ppm 7.50E+05 96.74% 22,250,200
Chlorine 1 ppm 1.15E+06 95.00% 21,850,000

 

Test Microorganism Test Substance Test Substance Concentration CFU/ml Percent Reduction Compared to Control at Time Zero Tot Kill
 E. coli
8739
Control Control 1.02E+07 N/A
KL-615 1 ppm 7.20E+06 29.41% 2,999,820
KL-1112 1 ppm 5.65E+06 44.61% 4,550,220
Chlorine 1 ppm 9.25E+06 9.31% 949,620

 

Test Microorganism Test Substance Test Substance Concentration CFU/ml Percent Reduction Compared to Control at Time Zero Tot Kill
S. enterica
10708
Control Control 2.46E+07 N/A
KL-615 1 ppm 2.35E+07 4.67% 1,148,820
2 ppm <5 >99.99% 24,600,000
KL-1112 1 ppm 1.45E+07 41.06% 10,100,760
2 ppm <5 >99.99% 24,600,000
Chlorine 1 ppm 2.67E+07 None None
2 ppm 2.16E+07 12.4% 3,050,400

 

Penicillium digitatum bench test Conducted by CA EPA.

Concentrations (mg/L) of sanitizer that inhibit 50% (EC50) or 99% (EC99) of germination of conidia of P. digitatum exposed to them for 10 min at 23 C. Germination was determined after incubation on potato dextrose agar for 18H. Concentrations and 95% confidence intervals (Cl) were estimated using Finney’s Probit analysis.

Chemical EC50 95% CL EC50 95% CL
Glutaraldehyde 1243 1116-1372 2774 2503-3157
Formaldehyde 1200 1023-1392 2502 2237-3140
Peroxide 676 610-745 1691 1537-1893
Chlorine 44.3 31.1-60.3 116.6 91.1-173.7
KL-615 9.4 4.9-16.5 25.3 17.7-56.1