Suspension Time Kill Testing: ASTM E 2315 : Testing Facility Operation 026.2
Test Microorganism | Test Substance | Test Substance Concentration | CFU/ml | Percent Reduction Compared to Control at Time Zero | Tot Kill |
L. monocytogenes 15313 |
Control | Control | 2.30E+07 | N/A | |
KL-AOP | 1 ppm | 7.50E+05 | 96.74% | 22,250,200 | |
KL-1112 | 1 ppm | 7.50E+05 | 96.74% | 22,250,200 | |
Chlorine | 1 ppm | 1.15E+06 | 95.00% | 21,850,000 |
Test Microorganism | Test Substance | Test Substance Concentration | CFU/ml | Percent Reduction Compared to Control at Time Zero | Tot Kill |
E. coli 8739 |
Control | Control | 1.02E+07 | N/A | |
KL-AOP | 1 ppm | 7.20E+06 | 29.41% | 2,999,820 | |
KL-1112 | 1 ppm | 5.65E+06 | 44.61% | 4,550,220 | |
Chlorine | 1 ppm | 9.25E+06 | 9.31% | 949,620 | |
Test Microorganism | Test Substance | Test Substance Concentration | CFU/ml | Percent Reduction Compared to Control at Time Zero | Tot Kill |
S. enterica 10708 |
Control | Control | 2.46E+07 | N/A | |
KL-AOP | 1 ppm | 2.35E+07 | 4.67% | 1,148,820 | |
2 ppm | <5 | >99.99% | 24,600,000 | ||
KL-1112 | 1 ppm | 1.45E+07 | 41.06% | 10,100,760 | |
2 ppm | <5 | >99.99% | 24,600,000 | ||
Chlorine | 1 ppm | 2.67E+07 | None | None | |
2 ppm | 2.16E+07 | 12.4% | 3,050,400 |
Penicillium digitatum bench test Conducted by CA EPA.
Concentrations (mg/L) of sanitizer that inhibit 50% (EC50) or 99% (EC99) of germination of conidia of P. digitatum exposed to them for 10 min at 23 C. Germination was determined after incubation on potato dextrose agar for 18H. Concentrations and 95% confidence intervals (Cl) were estimated using Finney’s Probit analysis.
Chemical | EC50 | 95% CL | EC50 | 95% CL |
Glutaraldehyde | 1243 | 1116-1372 | 2774 | 2503-3157 |
Formaldehyde | 1200 | 1023-1392 | 2502 | 2237-3140 |
Peroxide | 676 | 610-745 | 1691 | 1537-1893 |
Chlorine | 44.3 | 31.1-60.3 | 116.6 | 91.1-173.7 |
KL-AOP | 9.4 | 4.9-16.5 | 25.3 | 17.7-56.1 |